- William Asa Finley (1865-1872)
- Joseph Emery (1872 acting)
- Benjamin Lee Arnold (1872-1892)
- John Davidson Letcher (1892 acting)
- John McKnight Bloss (1892-1896)
- Henry B. Miller (1896-1897)
- Thomas Milton Gatch (1897-1907)
- William Jasper Kerr (1907-1932)
- George Wilcox Peavy (1932-1940)
- Frank Llewellyn Ballard (1940-1941)
- Francois Archibald Gilfillan (1941-1942 acting)
- August LeRoy Strand (1942-1961)
- James Herbert Jensen (1961-1969)
- Roy Alton Young (1969-1970 acting)
- Robert William MacVicar (1970-1984)
- John Vincent Byrne (1984-1995)
- Paul G. Risser (1996-2002)
- Timothy P. White (2002-2003 acting)
- Edward John Ray (2003-2020)
- F. King Alexander (2020-2021)
- Edward Feser (2021 acting)
- Rebecca Johnson (2021-2022 acting)